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Plasmid#140017PurposePlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT sfGFP 150TAG
Plasmid#154771Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-MGT4-d2EGFP_CMV-mCherry
Plasmid#213764PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#4-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CLGT3-d2EGFP_CMV-mCherry
Plasmid#213766PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertCLGT#3-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-C1-crRNA-cloning-EF1a-BA
Plasmid#204623PurposepiggyBac vector for crRNA cloning and expression. Blasticidin selectable.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Antares2-N1
Plasmid#120869PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertAkaluc
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-HD252
Plasmid#172656PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD252A/pegRNA-HD252B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR[dsxM]
Plasmid#131616PurposeExpresses a functional PuroR only in males and dsRed in both genders.DepositorInsertsUseTagsExpressionInsectMutationPromoterOpie2 and hr5Ie1Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H2B
Plasmid#133387PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnGW
Plasmid#133355PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP
Plasmid#124181PurposepiggyBac-based reporter (Puro) driven by the Pou5f1 enhancerDepositorInsertIRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP
Plasmid#124180PurposepiggyBac-based reporter (EGFP) driven by the Pou5f1 enhancerDepositorInsertEGFP-IRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster nanosAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZ10 PBREBAC-H2BHalo
Plasmid#91564Purposepiggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.DepositorInserthistone H2B-HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterEF1Available sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only