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PHF20 (3Q1J)
Plasmid#28278DepositorInsertPHF20 (PHF20 Human)
UseTagsHis with TEV cleavageExpressionBacterialMutationresidues 1-81.PromoterAvailable sinceApril 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QN1
Plasmid#61944PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSHPRH (SHPRH Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-RABGDI
Plasmid#28167PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB GDP dissociation inhibitor (PFL2060c Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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PP-CDPK2 (3PM8)
Plasmid#28272DepositorInsertPP-CDPK2 (PFF0520w Plasmodium falciparum)
UseTagsHisExpressionBacterialMutationContains CAD domain (residues 335-513).PromoterAvailable sinceApril 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15bHis6-pG-MNase
Plasmid#124886PurposeTo transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.DepositorInsertprotein G-MNase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCG-scr
Plasmid#229847PurposeExpresses wild-type Cas9 and scrambled gRNA.DepositorInsertguide RNA with scrambled sequence
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXR004_puroR
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ1-g1g2-K2
Plasmid#218217PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatibleDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK7-g7g8-K8
Plasmid#218220PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK3-g3g4-K4
Plasmid#218218PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK5-g5g6-K6
Plasmid#218219PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Senp2
Plasmid#175118PurposeLentiviral expression of V5-tagged mouse Senp2DepositorInsertSenp2 (Senp2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB-PE2
Plasmid#173902PurposeSB-transposon with constitutive expression of SpCas9 PE2DepositorInsertPE2
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
