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pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PFN1_Profilin
Plasmid#110028PurposeProtein expression and purification of PFN1_ProfilinDepositorInsertPFN1_Profilin (PFN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorInserthuman mature (delta19) ETFalpha (ETFA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1-Prig-3::tagBFP
Plasmid#165598Purposerig-3 promoter derive BFP expression in AVA head neurons.DepositorInsertPrig-3, tagBFP
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Frb-N-TEV
Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
iLID-mCl3-MAP7N
Plasmid#190167PurposeExpresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertiLID-mClover3-MAP7N
UseTagsiLID-mClover3ExpressionMammalianMutationaa 1-309 from human MAP7 delta175-213PromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
UseTagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s
Plasmid#108118PurposeRatiometric pH probe with enhanced brightness for mammalian cells expression.DepositorInsertSypHer3s
UseTagsratiometric YFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.DEST.puro
Plasmid#32682PurposeLentiviral cDNA/shRNA gateway destination vectorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
STK11 gRNA (BRDN0001146880)
Plasmid#75912Purpose3rd generation lentiviral gRNA plasmid targeting human STK11DepositorInsertSTK11 (STK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA_449
Plasmid#179093Purposenuclease Cas expressionDepositorInsertSpG
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2max-BSD
Plasmid#191103PurposeLentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD markerDepositorInsertNRCH-PEmax
UseCRISPR and LentiviralTagsExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9-NRCHPromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHUC-JEDI-1P
Plasmid#202619PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expressionDepositorInsertJEDI-1P
UseZebrafish expressionTagsExpressionMutationPromoterHuCAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6B
Plasmid#19884DepositorInserttrinucleotide repeat containing 6B (TNRC6B Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-Giantin
Plasmid#129630PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorArticleInsertmTurquoise2-Giantin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xMS2-hTR
Plasmid#154940Purpose4xMS2 stem-loop-hTR-500 bp genomic sequenceDepositorInsert4xMS2 stem-loop-hTR-500 bp genomic sequence
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
