Addgene

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pHcRed-CLCa

Plasmid

#196911

Purpose

visualization of clathrin-coated vesicle in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

Clathrin-Coated light chain

Use

Tags

HcRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3cIfm

Plasmid

#193778

Purpose

Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SchBE4max

Plasmid

#192135

Purpose

Expresses AncBE4max and nSchCas9

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

AncBE4max, nSchCas9

Use

Tags

Expression

Mammalian

Mutation

SchCas9 (D9A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13C^halo∆1235-1748

Plasmid

#187297

Purpose

Express VPS13C truncation mutant in mammalian cells

Depositor

Pietro De Camilli

Article

Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS13C truncation mutant (VPS13C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-24xopto-TetO-no01

Plasmid

#174887

Purpose

Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

24xopto-TetO

Use

Southern blot probe

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-T#1

Plasmid

#171515

Purpose

deletion of a genomic locus in T(Brachyury) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T (T Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-T#2

Plasmid

#171516

Purpose

deletion of a genomic locus in T(Brachyury) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T (T Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#1

Plasmid

#171503

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#2

Plasmid

#171504

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#1

Plasmid

#171505

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#2

Plasmid

#171506

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS

Plasmid

#192607

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-eGFP-P2A-CRY2-VPR

Plasmid

#192610

Purpose

Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

eGFP-P2A-CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

eGFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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