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Plasmid#176315PurposehU6-BpiI-Cas13bt3-DR (crRNA expression)DepositorInsertCas13bt3 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-mPGK-eGFP (beta-globin intron)
Plasmid#162513PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intronDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromotermPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIEx4-Cre
Plasmid#198280PurposeExpresses Cre in insect cellsDepositorInsertpIEx4-Cre
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-Cas9NG
Plasmid#203328PurposePlasmid for bacterial purification of codon optimized ABE8e-Cas9NGDepositorInsertABE8e-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Golgi
Plasmid#36205PurposeIn vivo visualization of golgi apparatus (can be used for colocalization studies)DepositorInsertGolgi targeting sequence (B4GALT1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
EcNR2 Strain
Bacterial Strain#26931DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV1)
Viral Prep#100054-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_MARV_GP
Plasmid#207292PurposeMammalian expression of Lake victoria Marburgvirus GP proteinDepositorInsertMARV_GP (GP Lake victoria Marburgvirus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-POLE2
Plasmid#160809PurposeExpress HA-tagged POLE2DepositorInsertHA-POLE2 (POLE2 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH86966
Plasmid#48075DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF69-2xCSTREP
Plasmid#136230PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF69DepositorInsertORF69
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
UseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_TBEV_PrME
Plasmid#207310PurposeMammalian expression of Tick-borne encephalitis virus PrME proteinDepositorInsertTBEV_PrME (POLY Tick-borne encephalitis virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human genome-wide lentiviral CRISPR gRNA library version 1
Pooled Library#67989PurposeHuman genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#209878PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddRFP A
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only