Addgene

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pShuttle

Plasmid

#16402

Purpose

Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#16402

Purpose

Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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mVenus C1

Plasmid

#27794

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus C1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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mVenus C1

Plasmid

#27794

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus C1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Tags

Expression

Mutation

Promoter

T7 (inactivated)

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Beta-Lactamase

Plasmid

#62729

Purpose

Expresses Beta-Lactamase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Beta-Lactamase

Use

Tags

His tag

Expression

Bacterial

Mutation

Deletion of periplasmic signal domain

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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Rosa26 mT/mG

Plasmid

#17787

Depositor

Liqun Luo

Article

Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

Insert

Rosa26 mT/mG

Use

Cre/Lox and Mouse Targeting

Tags

membrane tag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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Rosa26 mT/mG

Plasmid

#17787

Depositor

Liqun Luo

Article

Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

Insert

Rosa26 mT/mG

Use

Cre/Lox and Mouse Targeting

Tags

membrane tag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Article

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

mouse oxytocin

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCL1324

Plasmid

#217965

Purpose

Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Imp5 (IPO5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

plac

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Tags

Expression

Mutation

Promoter

KanR

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBR_attB(C31)_FRT

Plasmid

#183761

Purpose

phiC31-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge