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Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal His6-tagged intein SspExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Golgi-7
Plasmid#58018PurposeLocalization: Golgi Complex, Excitation: 555, Emission: 584DepositorInsertGolgi (B4GALT1 Human)
UseTagsmTagRFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC-SUR-YR
Plasmid#61768PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).DepositorInsertsSur gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromoterMagnaporthe oryzae ILV promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorInsertTGFB2 (TGFB2 Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myc-E-Syt1
Plasmid#66833PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt1 (ESYT1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FPP
Plasmid#203758PurposeEncodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertHRas c-term (HRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CAG-dLight1.1
Plasmid#111067PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.1
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-i-pep
Plasmid#47436PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
UseTagsGST tagExpressionBacterialMutationdeleted amino acids 1-43PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-PHLPP1 full length
Plasmid#37100PurposeExpresses HA tagged, full-length human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pS6-hα2M-H6
Plasmid#128495PurposeExpresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.DepositorInsertA2M (G27-S1468) (A2M Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-FLAG-Jhdm2a-H1122A
Plasmid#38140DepositorInsertJhdm2a-H1122A (Kdm3a Mouse)
UseTagsFLAGExpressionMammalianMutationprotein starts at aa115; H1122APromoterCMVAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K
Plasmid#110160PurposeExpression of human eEF2K in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDK4-3XFlag-pBabe
Plasmid#73558Purposevector encoding a fusion protein between CDK4 and 3 Flag tagsDepositorInsertCDK4 (CDK4 Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1(apf)
Plasmid#13846DepositorInsertJnk1a1(apf) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif Thr(183)-Pro-T…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE
Plasmid#198054PurposeAll-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloningDepositorInsertsrTetR(SE-G72P)-VP48-T2A-mCherry-BSD
3xFLAG-insert
UseLentiviral and Synthetic BiologyTags3xFLAGExpressionMammalianMutationPromoterTRE3G and pEFAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-Hs4E-T_AF
Plasmid#148687PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only