We narrowed to 21,655 results for: LEA

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-NL1

Plasmid

#173007

Purpose

For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-NL1

Tags

DHFR and EGFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pNSA-ARS-CRISPR-BlastR

Plasmid

#101009

Purpose

Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Reb_79 aka PAS620

Plasmid

#71225

Purpose

Reb_1 with RebA(Q91R)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA( G53A, Q91R)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Reb_80 aka PAS621

Plasmid

#71226

Purpose

Reb_1 with RebB(I67T, N84I)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(I67T, N84I)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Reb_65 aka PAS626

Plasmid

#71231

Purpose

Reb_1 with RebA(Y82A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, Y82A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF63

Plasmid

#13199

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF63

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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NFATc1_L (OZ543)

Plasmid

#28068

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
NFATc1_R (OZ544)

Plasmid

#28069

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
CT752

Plasmid

#233658

Purpose

Flp GFP Reporter; FRT-STOP-FRT-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CRY2olig-GP

Plasmid

#218918

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-GP

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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CRY2olig-GR

Plasmid

#218919

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-GR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-Plus-mCherry

Plasmid

#191231

Purpose

To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

AsCas12a-Plus-P2A-mCherry

Use

CRISPR

Mutation

E174R, R951K, R955A

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-kan

Plasmid

#108703

Purpose

Expression of an His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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