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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 7381 - 7400 of 12282 results

  8. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Expression
    Bacterial
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  9. TrxA-(A AND C) OR T-SpyTag

    Plasmid
    #246947
    Purpose
    Expresses multifunctional construct for (AND)OR-gated release of ThioredoxinA from materials in response to TEV OR (sortase eSrtA(2A9) AND HRV-3C) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246947-plasmid-map-sequence-id-494406

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  10. Neo-A OR T OR C-SpyTag

    Plasmid
    #246948
    Purpose
    Expresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246948-plasmid-map-sequence-id-489555

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  11. mGL-A AND T AND C-SpyTag

    Plasmid
    #246949
    Purpose
    Expresses multifunctional construct for AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV AND HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246949-plasmid-map-sequence-id-489547

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  12. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

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  13. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

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  14. NanoLuc-A AND T-SpyTag

    Plasmid
    #246946
    Purpose
    Expresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246946-plasmid-map-sequence-id-489552

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  15. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

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  16. phTERT-cp PS Intein tTA

    Plasmid
    #242032
    Purpose
    Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cp PS Intein tTA
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242032-plasmid-map-sequence-id-483130

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  17. Epac-S-H332

    Plasmid
    #228250
    Purpose
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228250-plasmid-map-sequence-id-451844

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  19. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  20. pSBtet-2xMARS-nGFP-puro

    Plasmid
    #205239
    Purpose
    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system
    Depositor
    Jeremy Baskin
    Publication
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Transposon
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    tight TRE promoter
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205239-plasmid-map-sequence-id-404740

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Showing: 7381 - 7400 of 12282 results