We narrowed to 14,041 results for: LEA

Showing: 7381 - 7400 of 14041 results

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9n

Use

CRISPR

Expression

Mammalian

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Publication

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV40_HA-mPACT

Plasmid

#120537

Purpose

protein expression

Depositor

Petr Svoboda

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PACT (Prkra Mouse)

Expression

Mammalian

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Publication

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1

Plasmid

#113943

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1

Depositor

Robert Campbell, Thomas Hughes

Publication

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1

Tags

6xHis

Expression

Bacterial

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbC

Plasmid

#114145

Purpose

Expresses ClbC-nHis

Depositor

Emily Balskus

Publication

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbC

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cyt-AKAR5

Plasmid

#114138

Purpose

Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Publication

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5 with C-terminal nuclear export signal (NES)

Expression

Mammalian

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.2s

Plasmid

#105069

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Publication

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.2s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Publication

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Publication

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pGEXndoE

Plasmid

#47714

Purpose

Expresses GST-EndoE from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Publication

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

Glutathione S-transferase

Expression

Bacterial

Promoter

tac

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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5C0M

Plasmid

#69962

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SGF29:JMC01N-E01:C207060 (SGF29 Human)

Tags

His and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO

Available since

Oct. 7, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem194 pmRFP-N2 (1389)

Plasmid

#62048

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tmem194 (TMEM194A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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mOrange2-PCNA-19-SV40NLS-4

Plasmid

#57971

Purpose

Localization: Nucleus, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mEmerald-PCNA-19-SV40NLS-4

Plasmid

#54223

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRCIscript-crhr2_TAL1A

Plasmid

#44236

Depositor

Karl Clark, Stephen Ekker

Publication

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1A

Use

Mrna transcription vector

Promoter

T3

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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