We narrowed to 16,633 results for: LEA

Showing: 7381 - 7400 of 16633 results

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3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.Ollas.S_mCherry-NLS

Plasmid

#178276

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.Ollas.S and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.Ollas_mCherry-NLS

Plasmid

#178259

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.NWS_mCherry-NLS

Plasmid

#178258

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.NWS and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shNts

Plasmid

#132717

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shNts (Nts Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2

Plasmid

#169216

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9

Plasmid

#163570

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
LCV2 V-4

Plasmid

#84807

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shDyn1

Plasmid

#132711

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(1) (Pdyn Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)

Plasmid

#110146

Purpose

Transformation and expression of Csy4 and cogDsRed proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Tags

cogRFP

Expression

Bacterial and Plant

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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LCV2 V-5

Plasmid

#84808

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)

Plasmid

#110145

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Tags

cogGFP

Expression

Bacterial and Plant

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-DroshaS302D

Plasmid

#62529

Purpose

Serine 302 of Drosha is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to aspartic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to aspartic acid

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E

Plasmid

#62528

Purpose

Serine 300 of Drosha is mutated to glutamic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300A

Plasmid

#62526

Purpose

Serine 300 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to alanine (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to alanine

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302A

Plasmid

#62527

Purpose

Serine 302 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to alanine (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to alanine

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only