We narrowed to 15,968 results for: LEA

1. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-BRD4ΔN-miRFP670-Cry2WT

   Plasmid

   #122439

   Purpose

   Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   Cry2WT and miRFP670

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX MLKL(wt)--2A-mCherry-puro

   Plasmid

   #231974

   Purpose

   Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-BG505.SOSIP-QES.i03.c01-8his

   Plasmid

   #111846

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE GFP-Ago2 S387A

   Plasmid

   #60654

   Purpose

   GFP-Ago2 S387A mutant lentiviral vector

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   S387A

   Promoter

   EF1a

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC588 - pAAV c-fos Nuc-iRFP

   Plasmid

   #59132

   Purpose

   An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Nuclear-localized Infrared Fluorescent Protein
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   mFos

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti GW V5 Eco/Dam mse LaminA

   Plasmid

   #182674

   Purpose

   Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

   Depositor

   Bas van Steensel

   Article

   Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

   Insert

   Lamin A (Lmna Mouse)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Promoter

   HSP

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TVBB C-term-mTurquoise2-Hygro

   Plasmid

   #169225

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Hygro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-N1-EXO1b-D173A

    Plasmid

    #111622

    Purpose

    Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

    Insert

    Exonuclease 1b D173A (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

    Plasmid

    #181956

    Purpose

    Genetically encoded FRET-based cAMP indicator fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
    

    Tags

    AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_CRFmut

    Plasmid

    #208659

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLRC1-NEK10p:NEK10-3XFLAG

    Plasmid

    #137030

    Purpose

    Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

    Depositor

    David Sabatini

    Article

    Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

    Insert

    NIMA-like kinase 10 (NEK10 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Promoter

    human NEK10 endogenous promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218914

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-S-HRV3C

    Plasmid

    #164567

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike - Furin site changed for HRV3C site
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    682-685 (furin site) replaced with HRV3C site (LE…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid

    #208960

    Purpose

    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid

    #208953

    Purpose

    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid

    #155013

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A227Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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