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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,852 results for: LEA

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Showing: 7381 - 7400 of 16852 results

  1. EPB41

    Plasmid
    #32040
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPB41 (EPB41 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    FERM domain only (amino acids 1-279)
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pEMS1098

    Plasmid
    #29029
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple202 (SLC6A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Available since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29029-plasmid-map-sequence-id-13444

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  3. pEMS1096

    Plasmid
    #29027
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple200 (SLC6A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Available since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29027-plasmid-map-sequence-id-13442

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  4. PIWIL2

    Plasmid
    #32042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIWIL2 (PIWIL2 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PAZ domain only (amino acids 386-533)
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  6. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R) (GYPA Staphylococcus aureus, Human)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  7. pYPQ239-RR

    Plasmid
    #108862
    Purpose
    FnCpf1 Gateway entry plasmid with N623R and K687R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R and K687R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108862-plasmid-map-sequence-id-274177

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  10. 4QPP

    Plasmid
    #61964
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available since
    Sept. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pCAG-HA-NLGN4 D396*

    Plasmid
    #59321
    Purpose
    Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation
    Depositor
    Peter Scheiffele
    Publication
    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLGN4 D396* (NLGN4X Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D396* nonsense mutation, rest of ORF deleted
    Promoter
    CAG
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    59321-plasmid-map-sequence-id-107090

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  12. PREX1

    Plasmid
    #32041
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PREX1 (PREX1 )
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    first PDZ domain only (amino acids Residues 607-7…
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid
    #223087
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223087-plasmid-map-sequence-id-447656

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  16. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid
    #205568
    Purpose
    RasAR negative-control mutant plasmid. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR(R89L)-NES (RAF1 Human, Synthetic)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Arg 89 mutated to Leu in Raf1 RBD region.
    Promoter
    CMV
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205568-plasmid-map-sequence-id-409421

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  17. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  18. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Publication
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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  19. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Publication
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    177335-plasmid-map-sequence-id-348601

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  20. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112289-plasmid-map-sequence-id-213521

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Showing: 7381 - 7400 of 16852 results