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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,243 results for: LEA

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Showing: 7381 - 7400 of 12243 results

  8. TrxA-(A AND C) OR T-SpyTag

    Plasmid
    #246947
    Purpose
    Expresses multifunctional construct for (AND)OR-gated release of ThioredoxinA from materials in response to TEV OR (sortase eSrtA(2A9) AND HRV-3C) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246947-plasmid-map-sequence-id-494406

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  9. Neo-A OR T OR C-SpyTag

    Plasmid
    #246948
    Purpose
    Expresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246948-plasmid-map-sequence-id-489555

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  10. mGL-A AND T AND C-SpyTag

    Plasmid
    #246949
    Purpose
    Expresses multifunctional construct for AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV AND HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246949-plasmid-map-sequence-id-489547

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  11. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

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  12. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

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  13. NanoLuc-A AND T-SpyTag

    Plasmid
    #246946
    Purpose
    Expresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246946-plasmid-map-sequence-id-489552

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  14. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

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  16. Epac-S-H326

    Plasmid
    #228242
    Purpose
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228242-plasmid-map-sequence-id-454214

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  17. Epac-S-H327

    Plasmid
    #228243
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228243-plasmid-map-sequence-id-451727

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  18. Epac-S-H329

    Plasmid
    #228245
    Purpose
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228245-plasmid-map-sequence-id-451790

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  20. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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Showing: 7381 - 7400 of 12243 results