We narrowed to 16,071 results for: LEA

1. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-BRCA1

   Plasmid

   #115491

   Purpose

   EGFP-BRCA1 expression vector. Parton clone BXY

   Depositor

   Rob Parton

   Article

   McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1009R (See depositor comments)

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-GRAB_CRF1.0

   Plasmid

   #208661

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-SMS1-TEV-Twin-Strep

   Plasmid

   #202528

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-ATF4 mScarlet NLS

   Plasmid

   #115969

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

   Plasmid

   #208952

   Purpose

   A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. OCT4-2A-eGFP-PGK-Puro

   Plasmid

   #31938

   Purpose

   Human OCT4 genomic targeting donor

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. elavl3:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218852

    Purpose

    D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

    Plasmid

    #210501

    Purpose

    expresses LAUNCHER component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ shSTING-Hsa

    Plasmid

    #128158

    Purpose

    Doxycyclin inducible shRNA knockdown of human STING gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shSTING Hsa (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINDUCER20-α-Syn

    Plasmid

    #92200

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for α-Syn

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC13N-iCAG.copGFP

    Plasmid

    #66578

    Purpose

    CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    copGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSEss-CHIg-mM_M18

    Plasmid

    #91729

    Purpose

    Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Promoter

    hEF1-HTLV prom

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55645

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV5, AAV8, and AAV9

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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