We narrowed to 16,443 results for: LEA

Showing: 7381 - 7400 of 16443 results

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pLIB-FLAG(N)-CDK9

Plasmid

#231719

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK9 (CDK9 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Rbx1:gs10:FRB:HA

Plasmid

#228534

Purpose

Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targeting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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6_T7-Lyn-mScarlet3

Plasmid

#225933

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

Lyn-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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7_T7-Src64B-mScarlet3

Plasmid

#225934

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

Src64B-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3-LactC2

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG-LRRC37B

Plasmid

#220790

Purpose

To express the human receptor LRRC37B in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

LRRC37B (LRRC37B Human)

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6-LSL (Cre dependent)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCi

Plasmid

#214038

Purpose

Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCi

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPCaspase

Plasmid

#214042

Purpose

Bacterial expression plasmid for PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase

Plasmid

#214043

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-N-term

Plasmid

#214044

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 1-153

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-C-term

Plasmid

#214045

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 154-666

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi

Plasmid

#214049

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-PCi

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PC-σ

Plasmid

#214037

Purpose

Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PC-σ

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

Plasmid

#208974

Purpose

Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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