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Plasmid#193594PurposeNFkB2 knockoutDepositorInsertsgNFkB2 guide 2 (NFKB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-Rac1-DN
Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-RhoAb-DN
Plasmid#109580PurposeMultisite gateway vector for 3' tagging with dominant negative RhoAbDepositorInsertRhoAb (rhoab Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mVenusN-Cavin3
Plasmid#108889PurposeExpression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertCavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
UseTagsmVenus1-155ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceMarch 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ML1Nmut_in_pEGFP-C1
Plasmid#67796PurposeMammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFPDepositorInsertML1N*2(7Q)-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG metap2
Plasmid#32004DepositorInsertmetap2 (Metap2 )
UseTagsFLAGExpressionMammalianMutationPromoterCMV, SP6Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3E-ING2
Plasmid#109554PurposeMultisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsert3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MLC2(CA)-IRES-GFP
Plasmid#133928PurposeBicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chainDepositorInsertEGFP and MLC2(CA) (MYL2 Human)
UseTagsExpressionMammalianMutationMLC2 T18D, S19DPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
Plasmid#162333PurposeLentiviral expression of dSpCas9-2xVP64 with blasticidin resistanceDepositorInsertdSpCas9-2xVP64
UseLentiviralTagsFLAGExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-ML1N*2
Plasmid#67678PurposeMultisite gateway entry clone for adding C-terminal fusions of ML1N*2DepositorInsertML1N*2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
UseTagsExpressionBacterialMutationA52VPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin1a-Clover
Plasmid#163852PurposeZebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPRDepositorInsertCavin1a (cavin1a Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPcrRNA_Lmo
Plasmid#126501PurposeL.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-mKate2
Plasmid#67671PurposeMultisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2DepositorInsertAPEX2-P2A-mKate2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only