-
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A
Plasmid#226572PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationR119APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen D188A
Plasmid#226573PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationD188APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen F191A
Plasmid#226571PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationF191APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant pLV-SigmaR1-mNeonGreen
Plasmid#226569PurposeEncodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)DepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226291PurposeLentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInserts2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR
UseLentiviralTags2xMyc-tdMCPExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-CEP192
Plasmid#227290PurposeDonor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)DepositorInsertCEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-V5-n2StayGoldC4 (JDW 1354)
Plasmid#224486PurposeGateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)DepositorInsertH2B-V5-n2StayGoldC4
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)
Plasmid#224537PurposeA Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pADepositorInsertIRES-(n2)StayGold(c4)-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 StayGold-E138D-T2A-mCherry
Plasmid#219833Purposeequimolar expression of Staygold-E138D and mCherry in budding yeastDepositorInsertStayGold-E138D-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-TRIP8b (constant) [N212/17R-2b]
Plasmid#206664PurposeMammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/17-2b.DepositorInsertanti-TRIP8b (constant) (Rattus norvegicus) recombinant Mouse monoclonal antibody (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-STAT4-P2A-mCherry
Plasmid#203853PurposeTet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertSTAT4 (STAT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-BACH1 Cas9-Resistant
Plasmid#199218PurposeLPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP::BACH1 (BACH1 Human)
UseTagsEGFPExpressionMammalianMutationsilient mutation at nucleic acid 177 C changed to…PromoterCMV D2ir promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA resistant ABHD16A-S176AS306A-mNG
Plasmid#195165Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-mNG
Plasmid#195166Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-mNG
Plasmid#195167Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-Halo
Plasmid#195168Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
UseTagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-Halo
Plasmid#195169Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
UseTagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK439 (Stag-3FLAG-P2A-BSD)
Plasmid#168234PurposeC-terminal tagging with Stag-3FLAGDepositorInsertStag-3FLAG-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[Khosta2 RBD]
Plasmid#190083Purposeexpress chimeric SARS-CoV spike with the Khosta-2 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only