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Plasmid#204020PurposeHomologous donor template for NFKB2 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB2-PGK-tdTomato
Plasmid#204021PurposeHomologous donor template for NFKB2 knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-min5UTR-hRluc-A60
Plasmid#182638PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase,
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-RPLP15UTR-hRluc-A60
Plasmid#182639PurposeTranscription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase
UseLuciferaseTagsExpressionMammalianMutationThe transcription is driven by a T7 promoter, pai…PromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-Bs3-sgRNA (BsaI)
Plasmid#188280PurposeMammalian expression, Genome editingDepositorInsertBs3-sgRNA
UseCRISPRTagsExpressionMammalianMutationnonePromoterU6Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-TalO8 (Blank-CoTrIP)
Plasmid#178303PurposeBlank CoTrIP plasmidDepositorTypeEmpty backboneUseTagsNAExpressionYeastMutationPromoterNAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH1002-Tier2(pUC ori)
Plasmid#169600PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Peft-pHCountdown-unc54
Plasmid#182304PurposeC. elegans plasmid encoding pH-Countdown with three intronsDepositorInsertpH countdown with 3 introns, with a Peft3 promoter and unc54 terminator
UseTagsExpressionWormMutationPromoterPeft-3Available sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-3'
Plasmid#110406PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2DepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(2)-CRY2-mCherry
Plasmid#160441PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(1)-CRY2-mCherry
Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(0)-CRY2-mCherry
Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) AcAc
Plasmid#111332PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAcPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pre-Ubc4 BrG pUC19
Plasmid#111322Purposefor transcription to Ubc4 pre-mRNADepositorInsertUBC4 (UBC4 Budding Yeast)
UseTagsExpressionBacterialMutationintron has branch-site A to G mutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1, 17
Plasmid#87893Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1, 17 (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1a
Plasmid#87902Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1a, 17
Plasmid#87900Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1a, 17 (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only