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Plasmid#165567PurposeType II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.DepositorInsertXbaI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-TthRT
Plasmid#165566PurposeDNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2DepositorInsertThermus thermophilus (Tth) RT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-KTHEAQpol
Plasmid#165542PurposeExonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.DepositorInsertKlenTaq1 polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SapIR
Plasmid#165537PurposeType II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.DepositorInsertSapI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-HindIIIR
Plasmid#165508PurposeType II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.DepositorInsertHindIII
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-NcoIR
Plasmid#165568PurposeType II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.DepositorInsertNcoI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SapIM2
Plasmid#165557PurposeSite-specific DNA methyltransferase 2; protects DNA from cleavage by SapIDepositorInsertM2.SapI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SapIM1
Plasmid#165547PurposeSite-specific DNA methyltransferase 1; protects DNA from cleavage by SapIDepositorInsertM1.SapI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-dromedNK
Plasmid#165579PurposeUsed for dNTP synthesisDepositorInsertdNK from drosophila
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRVM
Plasmid#165558PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by EcoRV.DepositorInsertM.EcoRV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Eco31IB
Plasmid#165527PurposeSite-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31IDepositorInsertEco31IB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Eco31IA
Plasmid#165517PurposeSite-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31IDepositorInsertEco31IA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Fpg
Plasmid#165541PurposeFormamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activitiesDepositorInsertFpg
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-NotIM
Plasmid#165518PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by NotIDepositorInsertM.NotI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-NcoIM
Plasmid#165578PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by NcoIDepositorInsertM.NcoI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Tlipol (exo-)
Plasmid#165523PurposeDNA polymerase; lacks 3' to 5' exonuclease activity.DepositorInsertTli DNA polymerase (exo-)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-XbaIM
Plasmid#165577PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by XbaIDepositorInsertM.XbaI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SfiIM
Plasmid#165538PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by SfiI.DepositorInsertM.SfiI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-9N7lig
Plasmid#165514Purpose9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C).DepositorInsert9 Degrees North DNA Ligase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Bst HF
Plasmid#165539PurposeHigh fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.DepositorInsertBst HF
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 1, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits