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Plasmid#119912PurposeMammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5Δ
UseTags6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZyxin-mMaple2
Plasmid#101150PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorInserthuman codon optimized mMaple2 fused to zyxin
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dronpa-Kras
Plasmid#182329PurposePlasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertDronpa-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARγ
Plasmid#119915PurposeMammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARγ
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-Actin/pEGFP-C1
Plasmid#51972Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actinDepositorInsertNano-lantern
UseTagsActinExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH16_AVR-Pib_100
Plasmid#121378PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH16_AVR-Pib_100
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH12-rn_00121641
Plasmid#121371PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH12-rn_00121641
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_PH14-rn_00186401
Plasmid#121369PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertPH14-rn_00186401
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-peroxisome/pcDNA3
Plasmid#51974Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomesDepositorInsertNano-lantern
UseTagsSKLExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-014
Plasmid#78702PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARζ
Plasmid#119918PurposeMammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARζ
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_CD156_AVR-Pib_70
Plasmid#121286PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertCD156_AVR-Pib_70
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_CD156_ms119_98
Plasmid#121285PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertCD156_AVR-Pimk1_98
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_br58_AVR-Pib_91
Plasmid#121272PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertbr58_AVR-Pib_91
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR32_AVR-Pib_97
Plasmid#121263PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR32_AVR-Pib_97
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR29_ms119_45
Plasmid#121236PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR29_AVR-Pimk1_45
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_gfsi_ms119_95
Plasmid#121334PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertgfsi_AVR-Pimk1_95
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_dig41_AVR-Pib_75
Plasmid#121287PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertdig41_AVR-Pib_75
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only