Addgene

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Showing: 7361 - 7380 of 54790 results

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p-G4PID-HA

Plasmid

#209639

Purpose

Expression G4PID-HA in mammalian cells

Depositor

Zhou Xiang

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-EEA1

Plasmid

#174452

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Use

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-mito

Plasmid

#174180

Purpose

Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

AcGFP with mitochondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-TagBFP

Plasmid

#214721

Purpose

Expresses green #2.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST53 PINK1 N-GFP

Plasmid

#13315

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-WT

Plasmid

#130700

Purpose

Expresses Beta-Globin WT NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad4

Plasmid

#37046

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad4 (SMAD4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
AWP-029

Plasmid

#213966

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Synthetic, Segatella bryantii)

Use

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1-HA

Plasmid

#24399

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Expression

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CUL4A

Plasmid

#19907

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Cullin 4A (CUL4A Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

K644R

Promoter

CMV

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdKatushka2-WPRE

Plasmid

#193344

Purpose

AAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pNANOG-Luc

Plasmid

#25900

Depositor

Ren-he Xu

Article

Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

Insert

NANOG promoter (NANOG Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2abcde

Plasmid

#80619

Purpose

pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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