-
Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-CUL5
Plasmid#19895DepositorInsertcullin 5 (CUL5 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-N
Plasmid#185482PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneUseTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTTP1A
Plasmid#91721Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…TagsExpressionMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT2-Flag
Plasmid#102623PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorInsertSirtuin 2 (SIRT2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-eGFP-beta 2 CP
Plasmid#13298DepositorInsertcapping protein beta 2 subunit (Capzb Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
Plasmid#104567PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activatorDepositorInserthuman codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
UseCRISPRTagsNLS-3xHA-VPRExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPlum-N1
Plasmid#54629PurposeLocalization: N1 Cloning Vector, Excitation: 590, Emission: 649DepositorTypeEmpty backboneUseTagsmPlumExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3K(1-10)
Plasmid#157993PurposeLentiviral vector of constitutive expression of mNeonGreen3K(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationG27D, D42E, M128I, K153M, T170IPromoterSFFVAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYP1-miniSOG-citrine
Plasmid#50971PurposeAAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) constructDepositorInsertSynaptophysin-miniSOG-citrine (Syp Rat)
UseAAVTagscitrine and miniSOGExpressionMutationPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-wtmrtfa-gfp
Plasmid#165037PurposeAAV-based delivery of wtMRTFA-GFP into cardiomyocytes in vivoDepositorInsertMrtfa (Mrtfa Mouse)
UseAAVTagsGFPExpressionMutationPromotercTNTAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
AAV-mDlx-jGCaMP8s-WPRE (AAV1)
Viral Prep#176755-AAV1PurposeReady-to-use AAV1 particles produced from AAV-mDlx-jGCaMP8s-WPRE (#176755). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8s-WPRE plasmid DNA. mDlx-driven expression of the calcium sensor jGCaMP8s in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsNoneAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (AAV1)
Viral Prep#179459-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (#179459). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE plasmid DNA. EF1a-driven, Cre-dependent soma and AIS-localized expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagscEGFP (Cre-dependent)Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B5
Plasmid#122555PurposeBlock 5 for AdenoBuilder genome assemblyDepositorInsertAdenovirus 5 genomic region 17998-25062
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only