Addgene

We narrowed to 19,042 results for: tes

Showing: 7341 - 7360 of 19042 results

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pDEST40-Arl13b11

Plasmid

#40873

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

His and V5

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
Flag-p53/pRK5

Plasmid

#39237

Depositor

Xiaolu Yang

Article

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

p53 (TP53 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shOrai1

Plasmid

#89933

Purpose

Knockdown human Orai1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 shRNA (ORAI1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Tetoffbidir -Alb-luc

Plasmid

#164078

Purpose

AAV vector with bidirectional promoter controls expression firefly Luciferase with TRE and Tet-off transactivator

Depositor

Shu Uin Gan

Article

Gan et al J Gene Med. 2019 Jan;21(1):e3067. doi: 1

Insert

Firefly Luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

Albumin

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pENTR223-EBNA3C

Plasmid

#198576

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pMMLV-mRFP1-P2A-Puro

Plasmid

#160228

Purpose

Expresses mRFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

mRFP1-P2A-Puro

Use

Retroviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

LTR

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEAK-bKal7

Plasmid

#145800

Purpose

expresses Kalirin-7 with the b-front in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

Insert

bKalirin-7 (Kalrn Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EIF2a

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFPI7

Plasmid

#83555

Purpose

Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CUP1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-FOXO3 6A

Plasmid

#24382

Depositor

Anne Brunet

Article

Greer et al J Biol Chem. 2007 Oct 12. 282(41):30107-

Insert

FOXO3 (FOXO3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T179A, S399A, S413A, S555A, S588A, and S626A

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Promoter

Available since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFSW GAP43-jRGECO1a P2A Syp-Halo

Plasmid

#185565

Purpose

Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

Depositor

Edwin Chapman

Article

Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

Insert

GAP43-jRGECO1a P2A Syp-Halo

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Use

Tags

FLAG and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Use

Tags

ssra

Expression

Bacterial

Mutation

Promoter

ipaH7.8p and rpsM

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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