-
Plasmid#68339PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.DepositorInsertEGFP
UseZebrafish transgenesisTagsExpressionMutationPromoterubiAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-DN (S25N)
Plasmid#101046PurposeExpresses HA-tagged human Rab11-DN (S25N) in mammalian cellsDepositorInsertRAB11 (RAB11A Human)
UseTagsHAExpressionMammalianMutationS25NPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pARF1(Q71L)-CFP
Plasmid#11385DepositorInsertADP Ribosylation Factor 1 (ARF1 Human)
UseTagsCFPExpressionMammalianMutationChanged Glutamine 71 to Leucine.PromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-RAB10
Plasmid#164631PurposeExpresses RAB10 in mammalian cells with a 2xmyc tagDepositorInsertRas-related protein Rab-10 (RAB10 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro_G-CEPIA1er
Plasmid#164590PurposeStable integration and expression of G-CEPIA1er in mammalian cellsDepositorInsertG-CEPIA1er
UseLentiviralTagsMycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
NSP3-FR-2Strep
Plasmid#210315PurposeExpresses SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertNSP3 (ORF1ab SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiHiFiCas9
Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70
Plasmid#62947PurposeCreating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-LexA::QFAD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateTagsExpressionMutationL22F F31SPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-SRGAP1
Plasmid#187269PurposeExpression of SRGAP1-EGFPDepositorInsertSRGAP1 (SRGAP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
-
REEP6-EGFP
Plasmid#210364PurposeExpresses REEP6 fused with EGFP in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin5
Plasmid#41916DepositorInsertCUL5 (CUL5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
PLK1-cjun-FRET-PACT biosensor
Plasmid#106907Purposecentrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)DepositorInsertPLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
UseTagsPACT domainExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70
Plasmid#62896PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pHAGE DN Cullin1
Plasmid#41911DepositorInsertCUL1 (CUL1 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only