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Plasmid#168310PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233293
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL75
Plasmid#168309PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233287
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL74
Plasmid#168308PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233281
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL73
Plasmid#168307PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233277
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL72
Plasmid#168306PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944231404
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY156: SUZ12 px330 sgRNA Cas9 plasmid
Plasmid#170792PurposeThis plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-wtBlc
Plasmid#168256PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondinglyDepositorInsertLeucine Zipper + C-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0.054
Plasmid#119572PurposeLevel 0 part. PromoterDepositorInsertPpetE
UseSynthetic BiologyTagsExpressionMutationRBS* added (22 bp) upstream of ATG. Deletion of n…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
UseTags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu-GFP 9A
Plasmid#113006PurposeP element vector for transposon insertion of gnu-GFP 9A into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutation9 CycB/CDK1 sites mutated to APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu-GFP WT
Plasmid#113005PurposeP element vector for transposon insertion of gnu-GFP WT into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu 9A
Plasmid#113000PurposeFor protein expression and purification of full-length Drosophila gnu 9ADepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutation9 CycB/CDK1 sites mutated to APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
full length png in pGEX4t
Plasmid#112974PurposeFor protein expression and purification of full-length Drosophila pngDepositorInsertpng (png Fly)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPJ169(JPUB_006769)
Plasmid#83835PurposeCarries NEW operon 5 finalDepositorInsertsKuste3340
Kuste3350
UseTagsExpressionMutationPromoterop5_3350_for and op5_BsaI_3340_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ170(JPUB_006771)
Plasmid#83833PurposeCarries NEW operon 6 finalDepositorInsertsKuste3346
Kuste3348
Kuste3349
UseTagsExpressionMutationPromoterop6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23109Available sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23117Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only