Addgene

We narrowed to 19,042 results for: tes

Showing: 7261 - 7280 of 19042 results

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-INPP4A

Plasmid

#171944

Purpose

Expresses INPP4A in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP4A (INPP4A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shTREX1

Plasmid

#127700

Purpose

Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shTREX1 (Trex1 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5

Plasmid

#65244

Purpose

Expression of HA tagged ERK5 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

A409T

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1b

Plasmid

#153976

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA recapping enzybe 1b (DCP1B Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Tags

Expression

Mutation

contains an altered Cre gene modified by the addi…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA1

Plasmid

#195827

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNA1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA1 (EBNA-1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-HA-2xMCP-KRAB

Plasmid

#126589

Purpose

Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-KRAB

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

Plasmid

#62903

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3B-IRES-Puro

Plasmid

#107345

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCHA1.1-MFSD12-V5

Plasmid

#149508

Purpose

Stable expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

David Root, John Doench

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPbU6_hdhfr/yfcu_Cas9

Plasmid

#216423

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEFBOS_CreIRESpuro

Plasmid

#183812

Purpose

Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

Depositor

Ed Stanley

Article

Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

Insert

Cre recombinase

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMALB

Plasmid

#161784

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

Depositor

Peter van Galen, John Dick

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Bidirectional minhCMV and hPGK

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Use

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only