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Plasmid#220455PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-Gal4
Plasmid#220450PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-LexAGAD
Plasmid#220458PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-p65AD
Plasmid#220457PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-Cre-miR122 TS
Plasmid#209784PurposeExpresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)DepositorInsertmiR122 ts
UseAAVTagsExpressionMutationPromotercTNTAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorInsertMutL homolog 1 (MLH1 Human)
UseLentiviralTagsExpressionMutationΔ754-756 dominant negative mutantPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro
Plasmid#209646PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3 proteins
Plasmid#204792PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro
Plasmid#209647PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro
Plasmid#209645PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100N-T26I&T77I
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro
Plasmid#209642PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro
Plasmid#209641PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-3xHA-Zeo
Plasmid#207740PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Zeo-3xHA
Plasmid#207707PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-3xHA
Plasmid#207696PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF3019
Plasmid#144495PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPOU2F1 (POU2F1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199195PurposeInsert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199196PurposeInsert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT57
Plasmid#204303PurposeExpresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only