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pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV iRFP L1-R5

Plasmid

#62156

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

H1, 7SK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 n.s shRNA L1-R5

Plasmid

#62135

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

non-silencing (scrambled) shRNA

Use

RNAi; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP R4-R3

Plasmid

#62141

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S100A, S495A and S511A

Plasmid

#33320

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S99A, S494A, S510A

Promoter

SV40

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
NHA-PAZ(aa1-480)

Plasmid

#21530

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

contain only aa1-480

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV tdTomato R4-R3

Plasmid

#62151

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3

Plasmid

#62169

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Luciferase

Use

Luciferase; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAC1-pCR8-dCas9VP48

Plasmid

#48214

Purpose

dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP48 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP48

Expression

Mutation

D10A;H840A

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP L1-R5

Plasmid

#62142

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBT3024

Plasmid

#122542

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_mt::CemOrange2

Use

Tags

MLSLRQSIRFFKPATRTLCSSRTLL

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV iRFP L5-L2

Plasmid

#62157

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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BRCA1-(1234-1443)

Plasmid

#35078

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

His

Expression

Mutation

Contains aa 1234-1443

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Use

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain pGEX

Plasmid

#38179

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain (BCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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