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Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJA4
Plasmid#19521DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp9 C287A
Plasmid#11819DepositorInsertCaspase 9 catalytically incompetent mutant (CASP9 Human)
UseTagsExpressionMammalianMutationC287A, catalytically incompetent mutant.PromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA2
Plasmid#19539DepositorInsertheat shock 70kDa protein 2 (HSPA2 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTU398
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
UseTagsHis6 and StrepExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC43
Plasmid#66565PurposesgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherryDepositorInsertssgRNA + 2XPP7
PCP-VP64 IRES mCherry
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SETDB1 ts1
Plasmid#115859PurposeSETDB1 knockdownDepositorInsertSETDB1 shRNA (SETDB1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy Flag GCN5
Plasmid#14106DepositorInsertGCN5 (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationE189G and K195NPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-BE4
Plasmid#108386PurposeMammalian expression vector for xCas9(3.6)-BE4DepositorInsertxCas9(3.6)-BE4
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H2
Plasmid#79513PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDSDepositorInsertLXR BETA (NR1H2 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
REEP5-EGFP
Plasmid#210363PurposeExpresses REEP5 fused with EGFP in mammalian cellsDepositorInsertREEP5 (REEP5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag hDPY30
Plasmid#15554DepositorInsertDPY30 (DPY30 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (W131G)
Plasmid#169724PurposeMammalian Expression of mouse SPOP W131GDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationW131GPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-1x
Plasmid#124679PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO mouse shRNA 1 rictor
Plasmid#21341DepositorInsertrictor shRNA 1 (Rictor Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab2 S20N
Plasmid#46780Purposeexpression of S20N Rab2a in mammalian cellsDepositorInsertRAB2 (RAB2A Human)
UseTagsmycExpressionMammalianMutationS20N dominant negativePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-BE3
Plasmid#108385PurposeMammalian expression vector for xCas9(3.6)-BE3DepositorInsertxCas9(3.6)-BE3
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only