Addgene

We narrowed to 22,286 results for: c-myc

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pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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HA-VHL 1-167-pRc/CMV

Plasmid

#22078

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-167 of wt VHL …

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pPPAT-11AA-2XStrep

Plasmid

#125680

Purpose

expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

the first eleven residues have been removed

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp7-HF

Plasmid

#157690

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp15-HF

Plasmid

#157722

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRTTO-codoptUL23-CStHA

Plasmid

#135099

Purpose

Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_115mut

Plasmid

#73068

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27 with AS box from …

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
HA-VHL 1-187-pRc/CMV

Plasmid

#22079

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-187 of wt VHL …

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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N-His-GvpC-ssrA

Plasmid

#153297

Purpose

Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)

Use

Tags

His-tag and ssrA tag

Expression

Bacterial

Mutation

1) N-terminal 6xHis tag flanked by 1x glycine res…

Promoter

T7

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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RF-118-UB-BRCA1

Plasmid

#78960

Purpose

Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

Depositor

Richard Fahlman

Article

Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

Insert

BRCA1 (BRCA1 Human)

Use

Tags

3x Flag and ubiquitin

Expression

Mammalian

Mutation

delta 1-1155

Promoter

CMV

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRTTO-codoptUL50-CStHA

Plasmid

#135105

Purpose

Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428(753)-VC

Plasmid

#123256

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Use

Tags

CD5 leader peptide and VC (Venus residues D155–K2…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; C-terminal Env re…

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα ST

Plasmid

#59919

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Tags

Expression

Mutation

changed sequence of the intracellular (C-terminal…

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only