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Plasmid#20005DepositorInsertDYRK2 (DYRK2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSMARCA4 guide 1
Plasmid#193604PurposeSMARCA4 knockoutDepositorInsertsgSMARCA4 guide 1 (SMARCA4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
UseTagsExpressionWormMutationPromoterpie-1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2616
Plasmid#91090PurposeModule C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
Plasmid#67668PurposeZebrafish expression vector for ubiquitous expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-STAT1
Plasmid#82722PurposeExpresses human STAT1DepositorInsertSTAT1 (STAT1 Human)
UseTags3xFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)
Plasmid#115789PurposeExpresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.DepositorInsertNDP (NDP Human)
UseTagsalkaline phosphatase and flagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2615
Plasmid#91078PurposeModule B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV5B+longBRD4
Plasmid#157792PurposeExpression of long isoform of BRD4DepositorInsertlong BRD4 (BRD4 Human)
UseTagsHis6ExpressionInsectMutationPromoterpolyhedronAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCS.DT-A
Plasmid#22730DepositorInsertDT-A
UseBac recombineeringTagsExpressionMutationThis is a gap-repair vector designed for retrievi…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX304-CAR-V5
Plasmid#82723PurposeExpresses V5 tagged coxsackievirus and adenovirus receptor (CAR).DepositorInsertcoxsackievirus and adenovirus receptor (CXADR Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX302-JSP1-V5 puro
Plasmid#87773PurposeExpresses V5 tagged human JSP1 (DUSP22)DepositorInsertJSP1 (DUSP22 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG2_Actin_2
Plasmid#109990PurposeProtein expression and purification of ACTG2_Actin_2DepositorInsertACTG2_Actin_2 (ACTG2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SLC39A14-GFP
Plasmid#104380PurposeExpresses SLC39A14/ZIP14 wild-type in mammalian cellsDepositorInsertSLC39A14 (SLC39A14 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only