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Showing: 7201 - 7220 of 14114 results

  4. Capping Protein

    Plasmid
    #89950
    Purpose
    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.
    Inserts
    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    Use
    Tags
    6*His
    Expression
    Bacterial
    Mutation
    V222I
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    89950-plasmid-map-sequence-id-183281

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  7. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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  8. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  11. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  14. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  15. t1-405(151-154AAAA)

    Plasmid
    #166131
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(151-154AAAA) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405 only. Contains 4 amino acid substitutions…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166131-plasmid-map-sequence-id-330282

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  16. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Use
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  19. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid
    #185484
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    185484-plasmid-map-sequence-id-363406

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  20. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid
    #185485
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    185485-plasmid-map-sequence-id-362670

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Showing: 7201 - 7220 of 14114 results