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Plasmid#212810PurposeConstitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cellsDepositorInsertH2B
UseTags8xHis and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo
Plasmid#212812PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-S32-KD
Plasmid#140299PurposeExpresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationlacks the UD regionPromoterCMVAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir144[309 nt]~451-CMV2-mir1
Plasmid#164680PurposeExpress miR-144[309 nt]~451 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir451~451-CMV2-mir1
Plasmid#164667PurposeExpress miR-451~451 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1
Plasmid#164682PurposeExpress miR-144[3072 nt]~451 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-mCherr
Plasmid#140307PurposeExpresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and UD regions fused to mCh…PromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S32-KD
Plasmid#140311PurposeExpresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationContains only the SH3, SH2, and KD regionsPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-S32
Plasmid#140303PurposeExpresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationlacks the KD regionPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationdeletion of 2 forkhead binding sites (bp# 395-497…PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Akt-FoxO3a-KTR-EGFP
Plasmid#125131PurposeFluorescent reporter for Akt activityDepositorInsertFOXO2 (FOXO3 Human)
UseTagsEnhanced green fluorescent protein (EGFP)ExpressionMammalianMutationFoxO3A 1-402 amino acidPromoterCAG promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP926
Plasmid#135001Purposem6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroRDepositorInsertpUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
UseLentiviral and Synthetic BiologyTags3xFLAG, Dam(R95A), and DpnI(aa146-254)ExpressionMammalianMutationDpnI domain truncated to aa146-254. Dam protein m…PromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M-Y419F-Y530F
Plasmid#140321PurposeExpresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298M-Y419F-Y530FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inpp5e
Plasmid#96808Purposecilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-nullDepositorInsert5HT6-YFP-Inpp5e
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSRE SSG
Plasmid#127692PurposePlasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Structural Genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEY17
Plasmid#191023Purposeflp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-18 CELE_Y48D7A.2 (flp-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inpp5e(D477N)
Plasmid#96809Purposephosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-nullDepositorInsert5HT6-YFP-Inpp5e(D477N)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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5HT6-YFP-Inp54p(D281A)
Plasmid#96813Purposephosphatase-dead control for 5HT6-YFP-Inp54pDepositorInsert5HT6-YFP-Inp54p(D281A)
UseTagsYFPExpressionMammalianMutationD281A inactivates phosphatase activityPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only