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Plasmid#194289PurposeMultisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQVDepositorInsertHyper7
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hyper7 in pT3TS-Dest
Plasmid#194296PurposeIn vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRLDepositorInsertHyper7
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-2PH-PLCd-mKate2
Plasmid#109500PurposeMuscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.DepositorInsertactc1b-2PH-PLCd-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
RA-NES
Plasmid#61019PurposeddRFP A-NESDepositorInsertddRFP A
UseTagsHindIII site and NES sequence MNLVDLQKKLEELELDEQQExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1W80A
Plasmid#64833PurposecDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drugDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation from Trp to Ala at position 80PromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
Plasmid#83925PurposeLentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastRDepositorInsertseGFP
Bsr
UseCRISPR and LentiviralTagsP2AExpressionMutationPromoterhUbCAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianMutationPromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
Plasmid#67668PurposeZebrafish expression vector for ubiquitous expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-WT
Plasmid#114037PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
UseTagsdimerization dependent fluorescent protein monomeā¦ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJB332
Plasmid#115949PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR-Gly9-DDX6 (contains SapI)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW122
Plasmid#115962PurposeORF insert for replicon MoCloDepositorInsertLvl0 5'-2xTetApt-mVenus-PEST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgMYC guide 2
Plasmid#193597PurposecMYC knockoutDepositorInsertsgMYC guide 2 (MYC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 Intergenic control guide 2
Plasmid#193585PurposesgRNA control; induces CAS9 cutting in an intergenic regionDepositorInsertsgRNA control
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only