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Plasmid#69238PurposeU6 driven SpCas9 sgRNA expression for KANK3 siteDepositorInsertKANK3 sgRNA (KANK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTGV-Express-Kan SMR5917
Plasmid#28038DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
UseTagskanMXExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-kanMX6
Plasmid#20757DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDZ415 (24MS2SL loxP-Kan-loxP)
Plasmid#45162DepositorInsert24xMS2 Stem Loop Cassette
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDZ416 (24PP7SL loxP-Kan-loxP)
Plasmid#45163DepositorInsert24xPP7 Stem Loop Cassette
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 KanR foundation lox casette
Plasmid#132388PurposeFoundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locusDepositorInsertG418 resistance
UseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterpromoterlessAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
BsaI-tagRFP-BsaI-pUC-kan
Plasmid#170771Purposetest plasmid for Golden Gate assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
Plasmid#173453PurposeContains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-kanMX6
Plasmid#20763DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
M4754 KanMX::HIS3 Disruptor Converter
Plasmid#51684PurposeYeast marker swap plasmid for converting KanMX3 to HIS3DepositorInsertHIS3 (HIS3 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Kan
Plasmid#188918PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)
Plasmid#173455PurposeContains mScarlet-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only