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Viral Prep#192552-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51084-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV PHP.eB)
Viral Prep#192552-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV5)
Viral Prep#84446-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV1)
Viral Prep#84446-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51082-AAV1PurposeReady-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (AAV1)
Viral Prep#51509-AAV1PurposeReady-to-use AAV1 particles produced from AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (#51509). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE plasmid DNA. hSyn-driven, Cre-dependent expression of cytoplasmic tdTomato and presynaptic EGFP (synaptophysin-fused). These AAV preparations are suitable purity for injection into animals.DepositorPromoterphSyn1TagstdTomato (cytoplasmic, Cre-dependent), EGFP (presynaptic, C…Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV8)
Viral Prep#84446-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51083-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (#51083). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato plasmid DNA. Cre-dependent GCaMP6f calcium sensor and physically separate nuclear tdTomato, under control of the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-NS1
Plasmid#175276PurposeAAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLSDepositorInsertNonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
UseAAVTagsNLS-dTomato (P2A cleavage)ExpressionMutationPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-R
Plasmid#60983PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomatoDepositorInsertdTomato
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWP89
Plasmid#61264PurposeIncP-based replicating vector expressing dTomato under tac promoter.DepositorInsertPtac-dTomato
UseSynthetic BiologyTagsExpressionBacterialMutationPromotertacAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hR
Plasmid#60997PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomatoDepositorInsertdTomato
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mR
Plasmid#60990PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomatoDepositorInsertdTomato
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENO1-dTom-NATr
Plasmid#52208PurposeConstitutive expression of Candida codon-optimized dTomato with NAT selectable markerDepositorInsertdTomato
UseTagsExpressionBacterial and YeastMutationcodon optimized for Candida speciesPromoterENO1Available sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1-Scr
Plasmid#133042PurposeSiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TripleColour-StAR
Plasmid#222695PurposeCompetition assayDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFP, miRFP703, dTomatoExpressionMammalianMutationPGK, TagBFP, dTomato without BsmBI cutsitePromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only