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Plasmid#173101PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
UseTagsmCeruleanExpressionBacterialMutationN78-S267, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP15-c021
Plasmid#173102PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
UseTagsmCeruleanExpressionBacterialMutationG287-N470, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase0
Plasmid#59122PurposeFAK fret control sensor without FERM domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
UseTagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase10
Plasmid#59123PurposeFAK fret control sensor without FERM domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoC
Plasmid#193809PurposeCMV overexpression of ChemoC in mammalian cell linesDepositorInsertChemoC
UseTags10xHisExpressionMammalianMutationmCerulean3:T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Synthetic, Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
ChromosomesMembranesandCytoskeleton
Plasmid#62450PurposeMXS_chaining vector with 4 cassettesDepositorInsertsCMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGDAP2 (GDAP2 Human)
UseTagsmCeruleanExpressionBacterialMutationM1-N231PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Rap1A FLARE
Plasmid#134926PurposeRap1A biosensorDepositorInsertRap1A, mCerulean3, RaIGDS RBD, YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A2 (MACROH2A2 Human)
UseTagsmCeruleanExpressionBacterialMutationK167-K372PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c002
Plasmid#173109PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
UseTagsmCeruleanExpressionBacterialMutationKH-M1-L192PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only