Addgene

We narrowed to 4,157 results for: Lta;

Showing: 701 - 720 of 4157 results

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-deltaNp53:WTp53

Plasmid

#49244

Purpose

expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN and p53 WT (TP53 Human)

Use

Tags

His and IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(mtSSB delta20)

Plasmid

#186280

Purpose

Expresses Human Mitochondrial SSB in E. coli

Depositor

Kenneth Johnson

Article

Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

Insert

Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

First 20 amino acids corresponding to probable mi…

Promoter

lambda

Available since

July 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

Plasmid

#180010

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB D185K/D186L

Plasmid

#132931

Purpose

p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag and pol D185K/D186L

Use

Lentiviral

Tags

Expression

Mutation

D185K/D186L

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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HDM-SARS2-Spike-delta21

Plasmid

#155130

Purpose

Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

Depositor

Jesse Bloom

Article

Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

Insert

SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon optimized to H. sapiens using IDT codon opt…

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-deltaLRR-cSSH

Plasmid

#220210

Purpose

Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

c.4-75del / p.2-24del

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

Plasmid

#220079

Purpose

SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-C389S-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

C389SdeltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB

Plasmid

#16067

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Basic Domain (aa1-44)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NMYC TRF2 deltaM

Plasmid

#16068

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Myb Domain (aa454-500)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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