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Plasmid#221518PurposeFlag-tagged biotinylation enzyme for co-expression of Lemd3DepositorInsertLEMD3 (LEMD3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEMD3_BirA_Cterm
Plasmid#221517PurposeFlag-tagged biotinylation enzyme for co-expression of Lemd3DepositorInsertLEMD3 (LEMD3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-NUP37 neo
Plasmid#220361PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37DepositorInsertNUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-CV-B3_3C
Plasmid#203499PurposeExpresses CV-B3 3C protease from a GAL promoter with a URA3 markerDepositorInsertCV-B3 3C protease (D1P32_gp1 )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-SV-A_3C
Plasmid#203474PurposeExpresses SV-A 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertSV-A 3C protease (Hk1_gp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HPeV_3C
Plasmid#203465PurposeExpresses HPeV 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHPeV 3C protease (polyprotein gene )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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V5-deltaUBA-short-UBASH3A
Plasmid#195405PurposeMammalian expression of human short-form UBASH3A lacking the UBA domain with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
K40R-short-UBASH3A-V5-6xHis
Plasmid#195407PurposeMammalian expression of human K40R short-form UBASH3A with V5-6xHis tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5-6xHisExpressionMammalianMutationK40RPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-long-UBASH3A
Plasmid#192087PurposeMammalian expression of human wild-type long-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
V5-W279A-long-UBASH3A
Plasmid#192085PurposeMammalian expression of human W279A long-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationW279APromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
V5-K202R-long-UBASH3A
Plasmid#192083PurposeMammalian expression of human K202R long-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationK202RPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorInsertWhi5-sg106 (WHI5 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-EYFP
Plasmid#158558PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD376
Plasmid#137022PurposeSapTrap mKate2-GLO donorDepositorInsertC. elegans GLO-optimized mKate2
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized mKate2ExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ Dja6_2
Plasmid#126895PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ Rac4
Plasmid#126896PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only