We narrowed to 16,052 results for: LEA

1. pDisplay-GRAB_CRF0.5

   Plasmid

   #208654

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ239-RR

   Plasmid

   #108862

   Purpose

   FnCpf1 Gateway entry plasmid with N623R and K687R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N623R and K687R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_NWS.Ollas.S_mCherry-NLS

   Plasmid

   #178279

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.Ollas.S and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_HSV.S.V5_mCherry-NLS

   Plasmid

   #178278

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.S.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_HSV.Ollas.V5_mCherry-NLS

   Plasmid

   #178277

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.Ollas.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_HSV.NWS.S_mCherry-NLS

   Plasmid

   #178273

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.NWS.S and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-LC3-K51A

   Plasmid

   #155265

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Lysine 51 to Alanine

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-LC3-R70A

    Plasmid

    #155264

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    Arginine 70 to Alanine

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. EF1a_DIO_R133C-HaloTag

    Plasmid

    #164064

    Purpose

    Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

    Depositor

    Nathaniel Heintz

    Article

    Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

    Insert

    MeCP2 Isoform 1 R133C (Mecp2 Mouse)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Drosha1-850

    Plasmid

    #62522

    Purpose

    C-terminal aa851-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 851-1374 deleted (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 851-1374

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Rat Alpha2 delta-1 mutMIDAS pMT2

    Plasmid

    #58730

    Purpose

    expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D259VSGS to AVAGA)

    Promoter

    SV40

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid

    #196519

    Purpose

    Expression of Ctdnep1_D67E-2xFlag

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead)

    Promoter

    CMV promoter; T7 promoter

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+CeGFP-Ctdnep1WTsiR

    Plasmid

    #196517

    Purpose

    Expression of Ctdnep1WT-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1WTsiR (CTDNEP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

20. mKO-EB3-7

    Plasmid

    #57838

    Purpose

    Localization: MT End Binding Protein, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Nishimura et al PLoS One. 2012;7(7):e41413. doi: 10.1371

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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