We narrowed to 16,027 results for: LEA

1. pRK5 beta4 1355T

   Plasmid

   #16038

   Depositor

   Filippo Giancotti

   Article

   Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

   Insert

   beta4 (ITGB4 Human)
   

   Expression

   Mammalian

   Mutation

   1355T: amino acids 1-1355

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pLCHKO_PDPR_exon_deletion_1

   Plasmid

   #155065

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJWK_VD_02

   Plasmid

   #158103

   Purpose

   5'UTR stem-loop luciferase reporter: -30kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop -30kcal/mol
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-mM₃O-IP

   Plasmid

   #46644

   Depositor

   Nobuaki Kikyo

   Article

   Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

   Insert

   mM₃O (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES Puro and mouse MyoD (aa 1-62)

   Expression

   Mammalian

   Mutation

   contains a fragment of mouse MyoD (aa1-62) fused …

   Available Since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

   Plasmid

   #46941

   Purpose

   Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with TALEN TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   embedded TALEN TS from 163-218, truncated 25 amin…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-HRV3C/D614G

   Plasmid

   #164568

   Purpose

   SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-HRV3C-D614G variant)
   

   Tags

   2X Strep-Tag II and 8X His tag

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRIME-CMV-GFP-shDyn2

   Plasmid

   #132712

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shDyn(2) (Pdyn Rat)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xNLS-NLP-cMyc-cMyc enAspCas12a

    Plasmid

    #182127

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc enAspCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_RBMX-eA3A-UdgX-NG-nCas9

    Plasmid

    #163551

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKO_Ptbp_ex8_3

    Plasmid

    #155083

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_HA.S.VSVg_mCherry-NLS

    Plasmid

    #178269

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_HA.HSV.VSVg_mCherry-NLS

    Plasmid

    #178261

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid

    #178260

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid

    #178255

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid

    #178246

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_C.HSV.S_mCherry-NLS

    Plasmid

    #178245

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid

    #178241

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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