We narrowed to 32,571 results for: LEA

1. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

   Plasmid

   #205278

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

   Plasmid

   #205278

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT3928

   Plasmid

   #113331

   Purpose

   control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Use

   Unspecified

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT3928

   Plasmid

   #113331

   Purpose

   control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Use

   Unspecified

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Tags

    TagRFP

    Expression

    Plant

    Promoter

    CaMV 35 promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Tags

    TagRFP

    Expression

    Plant

    Promoter

    CaMV 35 promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+MT-hToca-1-wt

    Plasmid

    #33030

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+MT-hToca-1-wt

    Plasmid

    #33030

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Csy4H29AS50C

    Plasmid

    #45029

    Depositor

    Jennifer Doudna

    Article

    Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

    Insert

    Csy4H29AS50C
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    H29A mutation to abolish catalytic activity and S…

    Promoter

    T7

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Csy4H29AS50C

    Plasmid

    #45029

    Depositor

    Jennifer Doudna

    Article

    Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

    Insert

    Csy4H29AS50C
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    H29A mutation to abolish catalytic activity and S…

    Promoter

    T7

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only