Addgene

We narrowed to 15,031 results for: grna

Showing: 7021 - 7040 of 15031 results

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pCas35

Plasmid

#82387

Purpose

sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas15

Plasmid

#82383

Purpose

sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas14

Plasmid

#82382

Purpose

sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas13

Plasmid

#82381

Purpose

sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

Plasmid

#177232

Purpose

Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik1_1st/sgSik2/sgSik3_1st

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

Plasmid

#177235

Purpose

Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgMark2/sgMark3/sgMark4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

Plasmid

#177236

Purpose

Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgSnrk

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

Plasmid

#177234

Purpose

Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNuak1/sgNuak2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

Plasmid

#177233

Purpose

Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgPrkaa1/sgPrkaa2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd

Plasmid

#177230

Purpose

Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgLkb1_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

Plasmid

#177227

Purpose

Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgLkb1_1st

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCG-scr

Plasmid

#229847

Purpose

Expresses wild-type Cas9 and scrambled gRNA.

Depositor

Natsuko Chiba

Insert

guide RNA with scrambled sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOliSpec5'

Plasmid

#228551

Purpose

Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

Depositor

Jeferson Gross

Insert

Truncated 5' region of aadA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pOliSpec3'

Plasmid

#228550

Purpose

Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

Depositor

Jeferson Gross

Insert

Truncated 3' region of aadA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSpec5'

Plasmid

#228549

Purpose

Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

Depositor

Jeferson Gross

Insert

Truncated 5' region of aadA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSpec3'

Plasmid

#228548

Purpose

Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

Depositor

Jeferson Gross

Insert

Truncated 3' region of aadA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

Plasmid

#177229

Purpose

Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgLkb1_2nd/sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

Plasmid

#177225

Purpose

Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgLkb1_1st/sgNeo1/sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

Plasmid

#177226

Purpose

Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgLkb1_1st/sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgLkb1_2nd-mU6-sgNeo1-hU6-sgNT

Plasmid

#177228

Purpose

Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgLkb1_2nd/sgNeo1/sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge