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Plasmid#203760PurposeFor Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertNAD KINASE2
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF2
Plasmid#200376PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-GT9
Plasmid#173376PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNN-NG dimer RVD at Position9
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppwd1 (PPWD1 Human)
UseTags(His)6-thrombinExpressionBacterialMutation473-646-W601APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1
Plasmid#182376PurposeDual expression construct encoding 3xFLAG and mito-mTFP1 from separate promotersDepositorInserts3xFlag
mito-mTFP1
UseTagsExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL315
Plasmid#168376PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOs09g0408550
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN095
Plasmid#173765Purposeexpress E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
UseTagshexa-histidine tagExpressionBacterialMutationdelete motif 3PromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_008
Plasmid#123761PurposeEncodes ERK Translocation Reporter (ERK KTR) as a Type 3a part to be used in the MTK systemDepositorInsertERKKTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_009
Plasmid#123762PurposeEncodes BxBI attP site as a Type 3a part to be used in the MTK systemDepositorInsertattP (BxBI)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_010
Plasmid#123763PurposeEncodes Phi31 attP site as a Type 3a part to be used in the MTK systemDepositorInsertattP (PhiC31)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_014
Plasmid#123766PurposeEncodes Kruppel Associated Box repression domain as a Type 3a part to be used in the MTK systemDepositorInsertKrab
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-HTNC
Plasmid#13763DepositorInsertTAT-NLS-Cre
UseBaculovirusTagsHis Tag, NLS, and TATExpressionBacterialMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-mTurquoise
Plasmid#172376PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertmTurquoise
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MGT1-d2EGFP_CMV-mCherry
Plasmid#213763PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15_attB(bxb)_lox
Plasmid#183764PurposeBxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(C31)_FRT
Plasmid#183761PurposephiC31-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only