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Plasmid#110071PurposeExpress C49S mutant of Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationCysteine 49 to SerinePromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IQCF2
Plasmid#110507PurposeFluorescent IQCF2DepositorInsertIQCF2 (IQCF2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8_yECitrine
Plasmid#110231PurposeCre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent proteinDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHSU71.2-2.2
Plasmid#110685PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-P2A-Puro
Plasmid#110849PurposeLentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-Puro
Plasmid#110850PurposeLentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQR-PGK-Puro
Plasmid#110855PurposeLentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGExpressionMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-PGK-Puro
Plasmid#110856PurposeLentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQR-P2A-GFP-PGK-Puro
Plasmid#110861PurposeLentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGExpressionMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-P2A-GFP-PGK-Puro
Plasmid#110862PurposeLentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-GFP-PGK-Puro
Plasmid#110863PurposeLentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.S221D
Plasmid#110355PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and mKO2ExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
mature ISG15 C-term. Domain (79-165, W123A)
Plasmid#110765PurposeBacterial expression of mutant proISG15 C-term. domainDepositorInsertproISG15 C-term. domain (79-165, W123A) (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationW123APromoterT7Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/hKGA.S286A
Plasmid#110357PurposeMammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tagDepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTagsV5ExpressionMammalianMutationS286APromotergagAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHSU62.2-2.2
Plasmid#110676PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHSU62.1-2.2
Plasmid#110675PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertPKS
UseTagsflagExpressionYeastMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHRASLS.2
Plasmid#110324PurposeTRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Homo sapiens, Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHRASLS.1
Plasmid#110323PurposeTRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Homo sapiens, Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3Q->E
Plasmid#110538PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationQ to E mutations in Nab3 low complexity domainPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316Nab3-11
Plasmid#110535PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationtwo missense mutations creating Nab3-11PromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only