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pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

Plasmid

#212384

Purpose

Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_29

Plasmid

#60272

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFP36L2 enhancer (ZFP36L2 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_31

Plasmid

#60273

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_34

Plasmid

#60276

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PNMA2 enhancer (PNMA2 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Lamin-A

Plasmid

#206027

Purpose

Expresses GFP-tagged Lamin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Lamin-A (LMNA Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_33

Plasmid

#60275

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GLIS3 enhancer (GLIS3 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-mTSG-GFAPCre

Plasmid

#100276

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Insert

GFAP promoter (Gfap, GFAP Human, Mouse)

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_27

Plasmid

#60270

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

C16orf80 enhancer (CFAP20 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_28

Plasmid

#60271

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CRY2 enhancer (CRY2 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_32

Plasmid

#60274

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

DGKB enhancer (DGKB Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_36

Plasmid

#60277

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

DNMT3A enhancer (DNMT3A Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_37

Plasmid

#60278

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ARHGAP26 enhancer (ARHGAP26 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS1096

Plasmid

#29027

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple200 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pNICBio2_WDR5:2-334

Plasmid

#211027

Purpose

bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR5:A2-C334 (WDR5 Human)

Use

Tags

MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE

Expression

Bacterial

Mutation

wild type

Promoter

T7

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Gamma

Plasmid

#177963

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )

Use

Tags

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETCON-HB50/design_43

Plasmid

#30270

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB50

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB56/design_34

Plasmid

#30276

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB56

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB4/design_2

Plasmid

#30271

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 16, 2011

Availability

Academic Institutions and Nonprofits only