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Plasmid#74748PurposeKillerOrange gene for bacterial expressionDepositorInsertKillerOrange
UseTagsHisTagExpressionBacterialMutationKillerRed with substitutions G3C Y66W D113S N145S…PromoterT5Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Mmp11
Plasmid#209520PurposeExpresses luciferase under control of Mmp11 promoter in transfected cellsDepositorInsertMmp11 promoter (Mmp11 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NfsB.zf1
Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionTagsExpressionMutationE28GPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-chlorR
Plasmid#199992PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorRDepositorInsertT7RNAP Circuit
UseTagsExpressionBacterialMutationcopFnullPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-GFP
Plasmid#66844PurposeTet-inducible expression of GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-HM4Di-YFP
Plasmid#209193PurposeCre-dependent expression of Hm4Di fused to YFP; ChemogeneticsDepositorInsertHm4Di
UseAAVTagsYFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyTagsExpressionMutationPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
UseTagsExpressionBacterialMutationPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP
Plasmid#157733Purposemammalian expression of untagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCruzHA SIRT1
Plasmid#10962DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-CasRX-P2A-EGFP-BGH PolyA
Plasmid#154004PurposeExpress CasRxDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40
Plasmid#154002PurposeEmpty SgRNA expression system matching CasRxDepositorInsertSgRNA expression system
UseCRISPRTagsExpressionMutationPromoterU6, EF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLP-pR-himar-apraR
Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport SREBP-1c dom neg
Plasmid#8885DepositorInsertSREBP-1c (Srebf1 Rat)
UseTagsExpressionMammalianMutationY305A amino acid substitution in the active versi…PromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pENTR-A1Up
Plasmid#16170DepositorInsertUbiquilin 4 (UBQLN4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN1-exon7-ESE1
Plasmid#126041PurposeIn-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN1 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
neurod1:CaMPARI
Plasmid#173479PurposeCaMPARI is driven by -5kb neurod1 promoter.DepositorInsertCaMPARI
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-C-8His-Flag
Plasmid#157678PurposeC-terminal tagging vectorDepositorInsert8His-Flag
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pPCK
Plasmid#133128Purposea recombinant plasmid, expressing pckA PEP-carboxykinase) geneDepositorInsertPEP-carboxykinase gene
UseTagsExpressionBacterialMutationPromoterPEP-carboxykinase promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVP2A-PIF6
Plasmid#73369PurposeExpresses Adeno-associated virus VP2 with PIF6 insert.DepositorInsertPIF6 (PIL2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PMXS-SLC2A3
Plasmid#72877Purposeexpresses SLC2A3 in mammalian cellsDepositorInsertSLC2A3 (SLC2A3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-FLAG-mPKM1
Plasmid#44240DepositorInsertPyruvate Kinase M1 (Pkm Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1u
Plasmid#13549DepositorInsertFLIP-E intramol WT (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
UseTagsExpressionBacterialMutationPromotermalate dehydrogenase promoterAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC flag-mRAP1 I312R
Plasmid#73138PurposeExpression of Flag-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BRAT1
Plasmid#62802PurposeExpresses human BRAT1 with an N-terminal HA tag in mammalian cellsDepositorInsertBRAT1 (BRAT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCK
Plasmid#49523PurposeKanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only