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Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_eCB2.0
Plasmid#164609PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in astrocytesDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterGfaABC1DAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit g2SE
Plasmid#49170PurposepHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0
Plasmid#164606PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST MTFR1L-V5
Plasmid#69822PurposeExpresses MTFR1L-V5 in mammalian cellsDepositorInsertMitochondrial fission regulator 1-like (MTFR1L Human)
UseRetroviralTagsv5ExpressionMammalianMutationPromotercmvAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInducer-TAZ
Plasmid#213587PurposeDoxycycline inducible expression of TAZ wild type cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-GLI2shR
Plasmid#136691PurposeInducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)DepositorInsertshRNA_humanGli2 (GLI2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorInsertTRIM28 (TRIM28 Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECE HA AMPKa1 WT
Plasmid#69504PurposeExpresses AMPK alpha1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR
Plasmid#136514PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vectorDepositorInsertCodon-optimized AcrIIA5
UseLentiviralTagsFLAG/NLSExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-FLCN
Plasmid#164981PurposeFLCN expressing vector for mammalian cellsDepositorInsertFLCN (FLCN Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Mouse, Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-GsCT
Plasmid#205516PurposeEndosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorInsertendo-GsCT (GNAS Synthetic, Human)
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette
Plasmid#113693PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.DepositorInsertSaCas9 gRNA Cassete
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
UseTagsNoneExpressionBacterialMutationPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iChR2++
Plasmid#98172Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChR2++ gene
UseTagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdPCP stdGFP
Plasmid#104099PurposeLentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.DepositorInsertstdPCP-stdGFP
UseLentiviralTagsN-terminal NLS, Two synonymous copies of GFP (C-t…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
All-in-one lenti-evoCDA-NG
Plasmid#200449PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB2
Plasmid#200982PurposeMammalian expression plasmid for myc-tagged EphB2DepositorInsertEphB2 (EPHB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc
Plasmid#113899PurposeMammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminaseDepositorInsertTetR-HA-NLS-P2A-BSD-Myc
UseLentiviralTagsHA-MycExpressionMutationPromoterCMV-MIEAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only