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pDONR223_MYC_WT

Plasmid

#82084

Purpose

Gateway Donor vector containing MYC , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MYC (MYC Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L1M

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH

Plasmid

#83907

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223-PANK4

Plasmid

#23419

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK4 (PANK4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLJM5-WT PHGDH

Plasmid

#83901

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-HK2

Plasmid

#23854

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK2 (HK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SPHK1

Plasmid

#23704

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SPHK1 (SPHK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Bcr/Abl P210-pLEF

Plasmid

#38158

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P210 (ABL1, BCR Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-PKM2

Plasmid

#23757

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKM2 (PKM Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TYK2

Plasmid

#23908

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TYK2 (TYK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_WT

Plasmid

#81857

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_WT

Plasmid

#82275

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-TNK1

Plasmid

#23894

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TNK1 (TNK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

Plasmid

#103864

Purpose

dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GSGGGGS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PLK1

Plasmid

#23867

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLK1 (PLK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

A3P

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_CHD2_WT

Plasmid

#81768

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PGK1

Plasmid

#23427

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PGK1 (PGK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Bcr/Abl P190-pLEF

Plasmid

#38159

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Use

Tags

GST

Expression

Mammalian

Mutation

T117M in c-ABL1

Promoter

SV40

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TBK1

Plasmid

#20648

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223-SYK

Plasmid

#23907

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SYK (SYK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZAP70

Plasmid

#23887

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAP70 (ZAP70 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge