Addgene

We narrowed to 13,199 results for: ung

Showing: 6901 - 6920 of 13199 results

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pAS-10 (bulge 9-13)

Plasmid

#40845

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–bulge 9-13

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAS-9 (bulge 9-11)

Plasmid

#40844

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–bulge 9-11

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Ubiquitin-WT

Plasmid

#17608

Purpose

Mammalian expression of HA tagged ubiquitin

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Expression

Mutation

Promoter

CMV

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Tags

Expression

Mutation

Promoter

SP6

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC0090

Plasmid

#181954

Purpose

Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xTOPFlash

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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HSA3_His

Plasmid

#202765

Purpose

human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA3

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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HSA1_His

Plasmid

#202763

Purpose

human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Hygro

Plasmid

#85134

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Blast

Plasmid

#85133

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Kras

Plasmid

#180571

Purpose

ddGFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Kras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Mar 1. ():.

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Hras

Plasmid

#180580

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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