Addgene

We narrowed to 8,097 results for: sgRNA

Showing: 6901 - 6920 of 8097 results

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pMXS-IRES-Blast SHMT2res

Plasmid

#106301

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Silent mutations destroy sgRNA targeting sites

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Tags

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone3

Plasmid

#162127

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPN007

Plasmid

#91575

Purpose

Express sgRNA targeting human BCL11A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BCL11A (BCL11A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp92

Plasmid

#157975

Purpose

pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgTAF12(human)#4.4

Plasmid

#105590

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12 HFD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_dNmeCas9

Plasmid

#120077

Purpose

dNmeCas9 with no sgRNA cassette lenti construct

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

dNmeCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp100

Plasmid

#157977

Purpose

pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2 V-1

Plasmid

#84804

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1162

Plasmid

#111098

Purpose

Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPN008

Plasmid

#91576

Purpose

Express sgRNA targeting human BCL11A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BCL11A (BCL11A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1272

Plasmid

#111107

Purpose

Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Neurog2

Plasmid

#99695

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)

Use

AAV and CRISPR

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD3-ovoD1

Plasmid

#111142

Purpose

Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

Depositor

Norbert Perrimon

Article

Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

Insert

pCFD3-ovoD1 (ovo Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM S297A

Plasmid

#169983

Purpose

Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, S297A

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgROSA

Plasmid

#105587

Purpose

lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting rosa26 locus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPN094

Plasmid

#91621

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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