Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 6881 - 6900 of 25278 results

  1. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

    Enlarge

  2. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

    Enlarge

  3. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

    Enlarge

  4. pET24a MDHNP_ARATH

    Plasmid
    #204238
    Purpose
    Bacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHNP_ARATH (AT5G58330 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include the chloroplastic transit sequen…
    Promoter
    Lac
    Availability
    Academic Institutions and Nonprofits only
    204238-plasmid-map-sequence-id-413341

    Enlarge

  5. pcDNA3.1-ARL16-His6

    Plasmid
    #216484
    Purpose
    Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    216484-plasmid-map-sequence-id-426903

    Enlarge

  7. pMINTNH3

    Plasmid
    #110074
    Purpose
    Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    His10 Tag - 3C cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110074-plasmid-map-sequence-id-264216

    Enlarge

  9. pMH-SFB

    Plasmid
    #99391
    Purpose
    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Type
    Empty backbone
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99391-plasmid-map-sequence-id-194202

    Enlarge

  17. pCm N-term MBP SMT3 NikJC199U

    Plasmid
    #174363
    Purpose
    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    174363-plasmid-map-sequence-id-342843

    Enlarge

  18. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

    Enlarge

  19. pET16b-Prp16

    Plasmid
    #111351
    Purpose
    protein purification
    Depositor
    Christine Guthrie
    Insert
    PRP16
    Use
    Tags
    N-terminally 10xHis-tagged proteins with a Factor…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    111351-plasmid-map-sequence-id-217673

    Enlarge

  20. ADH1pro_CBF1_SIR4

    Plasmid
    #138616
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    CBF1 (CBF1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138616-plasmid-map-sequence-id-275117

    Enlarge

Showing: 6881 - 6900 of 25278 results