We narrowed to 20,014 results for: 110

1. MYH11-NanoLuc-2A-tdTomato

   Plasmid

   #132569

   Purpose

   Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   Human MYH11 knockin homology arms (MYH11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ataxin-3 *VBM

   Plasmid

   #89976

   Purpose

   Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   mutated 282RKRR to 282HNHH

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG-IRF4

   Plasmid

   #58987

   Purpose

   MSCV based expression vector - co-expresses GFP and murine IRF4

   Depositor

   David Baltimore

   Article

   So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

   Insert

   Mouse IRF4 (Irf4 Mouse)
   

   Use

   Retroviral

   Tags

   Kozak sequence added

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.2/V5-DEST CDC42EP1 WT

   Plasmid

   #69819

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. InsRA-eGFP

   Plasmid

   #79795

   Purpose

   Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   eGFP extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MYC_pLX307

   Plasmid

   #98363

   Purpose

   Lentiviral expression of MYC

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2069

   Plasmid

   #141902

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOM20*-SBP-GFP

    Plasmid

    #120173

    Purpose

    Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Truncated TOM20: 1-30 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIBN-GFP-Sec61B

    Plasmid

    #104177

    Purpose

    Expresses fusion of CIB1 (1-170) with GFP and Sec61B

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CIBN-GFP-Sec61B (CIB1, SEC61B Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

    Plasmid

    #159576

    Purpose

    Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-M2L

    Plasmid

    #20926

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Inserts

    MYC (MYC Human)
    LIN28 (LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    CMV and IRES

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK9_pLX307

    Plasmid

    #98324

    Purpose

    Lentiviral expression of CDK9

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    CDK9 (CDK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF1704

    Plasmid

    #143308

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Tag2B EDD

    Plasmid

    #37188

    Depositor

    Charles Watts, Darren Saunders

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCh-KIF17*-strep

    Plasmid

    #120167

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 17 (KIF17 Human)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF17: 1-509 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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