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Showing: 6861 - 6880 of 14749 results

  3. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  7. pAAV-shMcu-1

    Plasmid
    #181868
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181868-plasmid-map-sequence-id-356032

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  8. pAAV-MCU-EGFP

    Plasmid
    #181866
    Purpose
    Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV and Adenoviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181866-plasmid-map-sequence-id-355942

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  15. pAAV-EFS-DIO-GRAB_CRFmut

    Plasmid
    #208663
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208663-plasmid-map-sequence-id-412146

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  19. pBactin-mOrange2-NEDD1-gTBD

    Plasmid
    #196858
    Purpose
    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-N-gTBD (NEDD1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196858-plasmid-map-sequence-id-391826

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  20. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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Showing: 6861 - 6880 of 14749 results