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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB512
Plasmid#74854PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
UseTags6xHis, E-tag, and myc-tagExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM553
Plasmid#68543PurposeNanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pY010 (pcDNA3.1-hAsCpf1)
Plasmid#69982PurposeExpresses humanized AsCpf1DepositorInserthAsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGPa-CMV-GFP-OSBP PH domain
Plasmid#58724PurposeExpresses GFP-Tagged OSBP PH domain in mammalian cellsDepositorInsertOxysterol-binding Protein PH domain (OSBP Human)
UseTagsGFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet (AAV1)
Viral Prep#131001-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarletAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-pcDNA3-PKCgamma-cys1Acys1B
Plasmid#21190DepositorInsertPKCgamma-cys1Acys1B (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…PromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.MaCPNS2
Plasmid#185137Purposenon-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-WT
Plasmid#64060PurposeEGFP fused at the N-terminus of tubulin; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag MKK6(K82A)
Plasmid#13519DepositorInsertMKK6(K82A) (MAP2K6 Human)
UseTagsFlagExpressionMammalianMutationLys-82AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6
Plasmid#170195PurposeMammalian expression of SARS-CoV-2 soluble spike trimer proteinDepositorInsertSARS-CoV-2 S (spike) (S SARS-CoV-2)
UseTagsHis6ExpressionMammalianMutationPromoterCMV51Available sinceNov. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F265-OKT3
Plasmid#190679PurposeEncodes an EGFRvIII-CD3 BITE protein (F265 clone)DepositorInsertAnti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NW30
Plasmid#133444PurposeCovalently circularized nanodisc NW30 for studying membrane proteins and viral entry.DepositorInsertNW30
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only
