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pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
UseTagsmRuby3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1 GSK3beta S9A HA
Plasmid#15994DepositorInsertGSK3beta (GSK3B Human)
UseTagsHAExpressionMammalianMutationS9A: constitutively activePromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-CAG-EGFP
Plasmid#153186PurposeCAG promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1441G
Plasmid#25082DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationArginine 1441 mutated to GlycinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-T2A-CopGFP
Plasmid#160501PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsCopGFPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
UseTagsLifeActExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-2xNLS-mTurquoise2
Plasmid#104053PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoterDepositorInsert2xNLS-mTurquoise2
UseAAVTagsNLSExpressionMutationPromoterGFAPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Epac1 CAAX
Plasmid#118312PurposeExpression of human Epac1DepositorInsertEpac1 CAAX (RAPGEF3 Human)
UseTagsGFPExpressionMammalianMutationC-terminal 20aa of KiRasPromoterCMVAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB316
Plasmid#68644PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NEK1_IDG-K
Plasmid#189871PurposeGateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK1 (NEK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mRuby2
Plasmid#99117PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2DepositorInsertDIO-mRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ERN2_IDG-K
Plasmid#189905PurposeGateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertERN2 (ERN2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-vox-EGFP
Plasmid#79970Purposemammalian GFP reporter plasmid, target site for Vika recombinaseDepositorInsert2 vox-sites flanking neo in frame with EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1+2 mutant
Plasmid#18975DepositorInsertLarge tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-KD-UniRapR-mCerulean-myc
Plasmid#45382DepositorInsertSrc-KD-UniRapR-mCerulean-myc
UseTagsmCerulean and mycExpressionMammalianMutationmSrc R388D (kinase dead), Y529FPromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2K930R-VSV
Plasmid#139346PurposeExpression of kinase-dead TYK2-K930R proteinDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-K930RPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
