We narrowed to 16,060 results for: grna

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pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

Plasmid

#79383

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Zhang 2.0 + scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

Plasmid

#154196

Purpose

Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sgRNA-BC1-A, sgRNA-BC1-C

Use

Lentiviral; Catch barcode activation

Expression

Mammalian

Promoter

hU6 and mU6

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

ALB_B358c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

Alb_A277c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEMPTY::sgRNA2

Plasmid

#165459

Purpose

Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

Depositor

Inigo Lasa

Article

Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

Insert

sgRNA2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

SP01

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

Plasmid

#228362

Purpose

mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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hCRISPRa-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

Pooled Library

#83981

Purpose

Human CRISPRa Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H1_1

Plasmid

#36342

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pRetrosuper USP28 shRNA-1

Plasmid

#15663

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 shRNA (USP28 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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MDH1-miR-30-PGK-GFP

Plasmid

#11379

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Insert

miR-30

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

Plasmid

#178994

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

Plasmid

#178992

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA-XMAS-1x

Plasmid

#164413

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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