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Plasmid#87781PurposeInducible expression of EGFP with a C-terminal NLS sequenceDepositorInserteGFP
UseLentiviralTags3xFLAG and NLSExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimEL
Plasmid#23103DepositorInsertpGSTag Bim EL (Bcl2l11 Mouse)
UseTagsGSTagExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Paxillin LD
Plasmid#186150PurposeFor expressing recombinant paxillin variants in bacteria.DepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationD5A; D146A; D232A; D267A; D301APromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPNT6-eGFP
Plasmid#71303PurposeThis vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expressionDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mis12 INCENP Δcen mCherry
Plasmid#45228DepositorInsertMis12 INCENP Δcen mCherry
UseTagsVSVGExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBlueRIP.OVA
Plasmid#69597Purposecontains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)DepositorInsertOVA (OVAL Chicken)
UseTagsExpressionMammalianMutationPromoterrat insulin promoter (RIP)Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
membrane-HsPLK1
Plasmid#185571Purposeto see protein dynamicsDepositorInsertPLK1 (PLK1 Human)
UseIn vitro transcription (mrna synthesis)Tagsmembrane targeting sequence from Neuromodulin (ML…ExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rap1b S179AS180A
Plasmid#118327PurposeExpression of bovine Rap1bDepositorInsertRap1b S179AS180A (RAP1B Bovine)
UseTagsGFPExpressionMammalianMutationS179AS180APromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
delta Zfp521
Plasmid#104190PurposeExpression of delta Zfp521 in mammalian cellsDepositorInsertZfp521 (Zfp521 Mouse)
UseTagsExpressionMammalianMutationdeletion of final 6 Zn fingersPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD375
Plasmid#91825PurposeCodon-optimized mKate2 donor for the SapTrap cloning systemDepositorInsertmKate2-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest472 SHP-1 S591D
Plasmid#21806DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 591 to Aspartic AcidPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTNS1
Plasmid#87801PurposeFor constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS NFATC1
Plasmid#19203DepositorInsertNFATC1 (NFATC1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pI12
Plasmid#24273DepositorInsertmodified adenovirus exons and introns
UseT7 for in vitro transcriptionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDZ536 pURA ADE3p PP7-PS-2x-yeGFP
Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA_381
Plasmid#179092Purposenuclease Cas expressionDepositorInsertIDT-Cas9_v2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_157
Plasmid#179090Purposenuclease Cas expressionDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_156
Plasmid#179089Purposenuclease Cas expressionDepositorInsertCas9-VRER
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_155
Plasmid#179088Purposenuclease Cas expressionDepositorInsertCas9-VQR
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_154
Plasmid#179087Purposenuclease Cas expressionDepositorInsertxCas9-3.7
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only