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Addgene
Showing: 6801 - 6820 of 8097 results

  1. pODN-mNG-CfANLN

    Plasmid
    #183837
    Purpose
    Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    Canis familiaris ANLN homology arms with mNeonGreen-linker
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183837-plasmid-map-sequence-id-362449

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  2. pgRNA_CV2

    Plasmid
    #167165
    Purpose
    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.
    Depositor
    Alexander Loewer
    Article
    Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167165-plasmid-map-sequence-id-330265

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  4. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160113-plasmid-map-sequence-id-325470

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Showing: 6801 - 6820 of 8097 results