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pMpGWB435
Plasmid#68700PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG.Myc-SARS2_3CL
Plasmid#200120PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cellsDepositorInsertSARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
UseTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK19_IDG-K
Plasmid#158175PurposeGateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK19 (CDK19 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB116
Plasmid#68570PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT010
Plasmid#64762PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB426
Plasmid#68691PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-Archer1-EGFP
Plasmid#60423PurposeLentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.DepositorInsertArcher1-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mTurquoise2
Plasmid#99115PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2DepositorInsertDIO-mTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-S127A
Plasmid#17794DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationSerine 127 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CB
Plasmid#12527DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele.PromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB331
Plasmid#68659PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N-CretrcintG
Plasmid#69572PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - N-CretrcintG
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only
